Mycoplasma contamination is a pervasive threat to cell culture work, affecting research integrity and experimental outcomes. Detecting mycoplasma early in the culture process is crucial for ensuring reliable results. The AffiZYME® MycoVisual Kit offers a rapid, simple, and reliable solution for routine mycoplasma screening, tailored for researchers who need accurate results without complicated equipment or procedures.
The Importance of Mycoplasma Detection
Mycoplasma contamination remains one of the most common and challenging issues in cell culture. It is often undetected for long periods due to its small size and lack of visible signs in the culture. This contamination can alter cell growth, affect gene expression, and lead to invalid experimental results. Early and routine detection is crucial in maintaining the quality and reproducibility of biological experiments.
AffiZYME® MycoVisual Kit: Key Features
1. Innovative LAMP Technology for Accurate Detection 🧬
The kit uses Loop-mediated Isothermal Amplification (LAMP) technology, a highly sensitive and specific method for detecting mycoplasma DNA. This technology amplifies DNA in a single, constant-temperature reaction, eliminating the need for complex PCR machines.
- No Special Equipment Required: Unlike traditional PCR methods, LAMP technology does not require expensive or specialized equipment, making it an ideal choice for labs with limited resources or for researchers seeking a cost-effective solution.
- High Sensitivity: LAMP can detect low levels of mycoplasma DNA, ensuring early detection before it impacts your cultures.
2. Rapid and Simple Procedure ⏱️
The AffiZYME® MycoVisual Kit offers fast results with a clear color change in just 60 minutes. The color shifts from purple-red to yellow as a visual indication of mycoplasma DNA presence, allowing researchers to quickly assess their cultures without complex analysis.
- Quick Screening: With results in under an hour, this kit is ideal for routine weekly screenings, ensuring that contamination is detected early and dealt with before it spreads.
- Colorimetric Visual Readout: The color change serves as a clear, intuitive indicator of mycoplasma contamination, making the process straightforward without the need for technical training.
3. Safe, Sealed-Tube Format 🧴
Safety is paramount when working with sensitive cell cultures. The AffiZYME® MycoVisual Kit comes in a sealed-tube format that eliminates the risk of contamination during testing.
- Prevents Cross-Contamination: The sealed-tube system ensures that no external contaminants interfere with the results, maintaining the integrity of your samples and ensuring accurate detection.
- Minimal Handling: With its easy-to-use design, the kit minimizes the risk of mishandling, which could lead to false results or contamination.
4. Ideal for Routine Screening 🗓️
The AffiZYME® MycoVisual Kit is designed for regular use, making it an excellent choice for labs that require frequent monitoring of their cell cultures. By incorporating this kit into your routine screening protocol, you can significantly reduce the risk of mycoplasma contamination affecting your experiments.
- Peace of Mind: Regular screenings with the AffiZYME® MycoVisual Kit ensure that your cultures remain uncontaminated, providing peace of mind and allowing you to focus on your research.
Why Choose the AffiZYME® MycoVisual Kit?
- Ease of Use: No need for specialized equipment or training — simply follow the easy instructions for reliable results.
- Cost-Effective: Affordable for both small and large-scale labs, with no hidden costs for reagents or equipment.
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Trusted by Researchers: This kit is trusted by scientists worldwide for its accuracy, speed, and simplicity.
How to Use the AffiZYME® MycoVisual Kit
- Sample Preparation: Add a sample of the cell culture supernatant to the sealed tube.
- Incubation: Incubate the tube at the recommended temperature for 60 minutes.
- Read Results: After incubation, observe the color change. Purple-red indicates no contamination, while yellow indicates the presence of mycoplasma DNA.
Where to Buy
The AffiZYME® MycoVisual Kit is available for purchase through AffiGEN your trusted partner in molecular diagnostics and life science research solutions. Ensure your lab’s success by keeping it free from mycoplasma contamination with the AffiZYME® MycoVisual Kit.
Conclusion
Routine mycoplasma detection is essential for maintaining the integrity of your cell cultures and ensuring the reliability of your research. The AffiZYME® MycoVisual Kit provides a fast, cost-effective, and reliable method for routine screenings, offering peace of mind and protecting your valuable cell cultures from contamination.
With its simple colorimetric readout, no special equipment, and sealed-tube format, the AffiZYME® MycoVisual Kit is an essential tool for researchers dedicated to achieving accurate and reproducible results. Trust AffiZYME® to keep your cell cultures safe, and your research on track.