pCDFDuet- 1 Plasmid - 2ug

Plasmid Name:

pCDFDuet™-1

Plasmid Overview

• Type: Bacterial Expression Vector
• Size: 3,781 bp
• Promoter: AmpR (Note: This might be an error; pCDFDuet™-1 typically uses the T7 promoter and confers streptomycin resistance—please verify)
• Cloning Method: Restriction Enzyme-based Cloning
• Expression Level: Unknown

Selectable Marker

• Bacterial Resistance: Streptomycin

Fusion Tags

• 5′ Terminal (Both Sites): N-Term
• 3′ Terminal (Both Sites): C-Term
• Tag 1: 6×His
• Tag 2: S15

Sequencing Primers

Site 1:

• 5′ Primer: ACYCDuetUP1 – GGATCTCGACGCTCTCCCT
• 3′ Primer: DuetDOWN1 – GATTATGCGGCCGTGTACAA

Site 2:

• 5′ Primer: DuetUP2 – TTGTACACGGCCGCATAATC
• 3′ Primer: T7term – GCTAGTTATTGCTCAGCGG