pDONR221- Kan - 2ug

Vector Overview

• Backbone Name: pDONR221
• Manufacturer: Invitrogen
• Backbone Size (without insert): 2,790 bp
• Vector Type: Gateway® Entry Vector

Bacterial Propagation

• Host Strain: E. coli DH5α
• Antibiotic Resistance: Kanamycin (50 μg/mL)
• Growth Temperature: 37 °C
• Copy Number: High copy

Insert Information

• Gene/Insert Name: Enhanced Green Fluorescent Protein (EGFP)
• Insert Size: 700 bp

Cloning Details

• Cloning Method: Gateway® Recombination Cloning Technology
• 5′ Sequencing Primer: M13pUC-forward
• 3′ Sequencing Primer: M13-reverse