Mycoplasma contamination in cell cultures is an ongoing challenge in biological research. While visual screening methods, such as those provided by the AffiZYME® MycoVisual Kit, can provide initial indications of contamination, quantitative confirmation is necessary to ensure accuracy. The AffiPCR® MycoDetect Kit steps in here, offering high-sensitivity, real-time PCR testing that confirms contamination with precision and reliability.
Why Quantitative Confirmation Matters
Mycoplasma contamination can have a significant impact on the quality of your research and experimental results. Early detection is critical, but it’s equally important to quantify the level of contamination. Quantitative PCR (qPCR) ensures the presence of mycoplasma DNA is not just detected but also measured with great accuracy, allowing for better-informed decisions in lab management and experimental protocols.
The AffiPCR® MycoDetect Kit is specifically designed to offer high sensitivity and rapid results, providing quantitative confirmation in under 2 hours. Let’s take a closer look at why this kit is an essential tool for every research lab.
AffiPCR® MycoDetect Kit: Key Features
1. High Sensitivity for Accurate Detection 🧬
With the ability to detect as few as 10 copies of mycoplasma DNA, the AffiPCR® MycoDetect Kit ensures that even minimal contamination can be identified and quantified. This high sensitivity is essential for catching contamination before it becomes a significant issue, offering early intervention in a way visual screening simply can’t.
- Detects Low Mycoplasma DNA Loads: Even if your cultures are showing only traces of contamination, this kit will catch it with high specificity.
- Ideal for Critical Applications: When working on sensitive experiments, confirming that your cultures are free of mycoplasma is crucial.
2. Rapid Results in Under 2 Hours ⏱️
The AffiPCR® MycoDetect Kit delivers fast results, providing quantitative confirmation in under 2 hours. This allows researchers to act quickly in managing contamination without unnecessary delays, keeping their work on track and minimizing the risk of spreading contamination in cultures.
- Quick Turnaround: Results in under 2 hours, so you can take immediate action if contamination is detected.
- Efficient Workflow: Helps streamline your lab workflow by providing rapid, reliable results.
3. Ct-Based Detection for Precision 📊
The AffiPCR® MycoDetect Kit utilizes Ct (cycle threshold)-based detection, a standard method in qPCR testing that offers precise results. A Ct < 35 indicates a positive result, meaning mycoplasma DNA is present in the sample. This easy-to-understand threshold ensures researchers know exactly what to expect from their test.
- Clear and Actionable Results: A Ct < 35 result confirms contamination, providing a definitive, actionable result.
- Quantification: Not only is the contamination detected, but the kit also helps quantify the level of contamination, allowing you to gauge its potential impact on your work.
4. Compatible with Most qPCR Platforms 🧪
The AffiPCR® MycoDetect Kit is designed to be compatible with most standard qPCR platforms, ensuring that researchers can seamlessly integrate it into their existing setups without the need for specialized equipment.
- Easy Integration: Compatible with leading qPCR platforms, reducing setup time and effort.
- No Additional Tools Required: The kit works with your lab’s current PCR system, making it easy to implement.
5. No DNA Extraction Required 🚫
One of the biggest advantages of the AffiPCR® MycoDetect Kit is that no DNA extraction is needed. This feature significantly reduces the processing time and complexity of your workflow.
- Simplified Process: Skip the DNA extraction step, saving valuable time and effort.
- Less Room for Error: By eliminating the extraction process, you reduce the chances of introducing errors or contamination into your samples.
6. Built-in Positive and Negative Controls ✅❌
To ensure the accuracy and reliability of results, the AffiPCR® MycoDetect Kit includes built-in positive and negative controls. These controls ensure that the test works correctly and that any potential issues with the reaction are identified right away.
- Confidence in Results: Positive and negative controls provide reassurance that the assay is functioning correctly.
- Error Detection: If something goes wrong, the controls will help pinpoint the issue.
How to Use the AffiPCR® MycoDetect Kit
- Prepare Your Sample: Add the sample from your cell culture to the reaction mix.
- Run the PCR: Set up the reaction on your qPCR platform, following the included protocol.
- Analyze Results: Review the Ct values. A Ct < 35 indicates a positive result for mycoplasma contamination.
Why Choose the AffiPCR® MycoDetect Kit?
- Highly Sensitive: Detects even the smallest amounts of mycoplasma DNA.
- Fast Results: Get results in under 2 hours, keeping your research moving forward.
- No DNA Extraction: Save time and reduce errors by skipping DNA extraction.
- Reliable and Accurate: Built-in controls and Ct-based detection ensure precision.
Where to Buy
The AffiPCR® MycoDetect Kit is available through AffiGEN, a trusted provider of life science research solutions. Keep your lab free from mycoplasma contamination with the AffiPCR® MycoDetect Kit, and ensure your results are accurate and reproducible.
Conclusion
For researchers who require precise, quantitative confirmation of mycoplasma contamination, the AffiPCR® MycoDetect Kit is the gold standard. With its high sensitivity, rapid results, and compatibility with most qPCR platforms, this kit ensures that you can detect, quantify, and confirm mycoplasma contamination quickly and reliably. Trust the AffiPCR® MycoDetect Kit to provide the actionable insights you need to maintain the quality of your research.