Mycoplasma contamination can cripple even the most advanced research, and once contamination is confirmed, the focus shifts to restoring clean and uncontaminated cultures. The AffiCLEAN® MycoX Removal Mix is a non-toxic, effective solution designed to remove mycoplasma contamination without damaging valuable cell lines. With over 70 strains of mycoplasma susceptible to its action, this concentrated reagent provides a reliable way to recover contaminated cultures.
Why Mycoplasma Removal Matters
Once mycoplasma contamination has been confirmed in a cell culture, researchers face the daunting task of eliminating the contamination without compromising the integrity of their work. Mycoplasma contamination can cause cell growth abnormalities, affect gene expression, and lead to unreliable experimental results. Restoring contaminated cultures is not just about removing the mycoplasma but doing so without disrupting ongoing research.
The AffiCLEAN® MycoX Removal Mix enables labs to restore contaminated cultures quickly and efficiently, allowing researchers to continue their work with confidence.
AffiCLEAN® MycoX Removal Mix: Key Features
1. Non-Toxic to Common Mammalian Cell Lines 🐇
The AffiCLEAN® MycoX Removal Mix is designed to be non-toxic to common mammalian cell lines, including CHO, HEK293, and Vero cells. This ensures that while it works to eliminate mycoplasma, it does not negatively impact the viability or health of the cells being treated.
- Safe for Routine Use: This reagent is suitable for most mammalian cell lines, making it versatile for a wide range of laboratory settings.
- Preserves Cell Health: While removing mycoplasma, the reagent ensures that your cells remain healthy and capable of continued use in experiments.
2. Fast and Effective Mycoplasma Removal ⏳
The AffiCLEAN® MycoX Removal Mix works effectively within 2–3 weeks, typically after 4–6 passages of the culture. This quick turnaround minimizes disruption to your workflow while ensuring that mycoplasma contamination is eliminated.
- Rapid Action: Effective removal in just a few weeks, allowing you to regain the integrity of your research cultures.
- Minimal Passages Needed: The reagent works during routine passages, saving you time and effort compared to alternative methods.
3. Simple 1:1000 Dilution into Culture Media 💧
The AffiCLEAN® MycoX Removal Mix is easy to use with its 1:1000 dilution into culture media. This simplicity reduces the need for complicated procedures and allows researchers to quickly incorporate the reagent into their existing workflows.
- Easy to Implement: A straightforward dilution ensures quick integration into lab protocols with minimal disruption.
- No Need for Additional Complex Steps: The single-step dilution makes it user-friendly, even for labs with limited experience in mycoplasma removal.
4. No Need to Discard Valuable Cell Lines 🧪
One of the greatest advantages of the AffiCLEAN® MycoX Removal Mix is that it allows researchers to retain their valuable cell lines. Unlike other methods that require the disposal of contaminated cultures, this reagent eliminates mycoplasma without forcing researchers to discard their critical cell stocks.
- Protect Your Research: There’s no need to throw away precious cell lines, which may have taken years to develop.
- Cost-Effective: By saving your cell lines, you avoid the cost and time involved in creating new cultures from scratch.
5. Over 70 Strains of Mycoplasma Targeted 🔬
The AffiCLEAN® MycoX Removal Mix is effective against over 70 strains of mycoplasma, making it a comprehensive solution for a wide variety of mycoplasma contaminants. This broad-spectrum activity ensures that no matter the strain, your cultures are effectively cleared of contamination.
- Wide-Ranging Effectiveness: Capable of handling most common mycoplasma strains found in cell cultures.
- Comprehensive Protection: The multi-strain efficacy ensures that you won’t be caught off guard by a contamination you weren’t anticipating.
How to Use AffiCLEAN® MycoX Removal Mix
- Dilute the Reagent: Add 1 part AffiCLEAN® MycoX Removal Mix to 1000 parts culture media.
- Incubate and Passage: Pass the treated culture through 4–6 passages, allowing the reagent to work effectively.
- Monitor for Results: Within 2–3 weeks, observe the cultures for the absence of mycoplasma contamination and the continued health of your cell lines.
Why Choose AffiCLEAN® MycoX Removal Mix?
- Safe and Non-Toxic: Works effectively without damaging your cells.
- Fast and Efficient: Restores your cultures in as little as 2–3 weeks.
- Simple to Use: Easy 1:1000 dilution integrates seamlessly into your lab protocol.
- Protects Valuable Research: No need to discard your cell lines — recover them and continue your work.
Where to Buy
The AffiCLEAN® MycoX Removal Mix is available through AffiGEN, a trusted supplier of advanced life science solutions. Keep your cell cultures clean and uncontaminated with AffiCLEAN® MycoX Removal Mix, and get back to your groundbreaking research with confidence.
Conclusion
For researchers dealing with mycoplasma contamination, the AffiCLEAN® MycoX Removal Mix offers a non-toxic, effective solution to restore valuable cell cultures. With its broad-spectrum activity, simple dilution, and minimal passage requirements, it’s the ideal tool for researchers looking to eliminate mycoplasma contamination and preserve their valuable cell lines.
Trust AffiCLEAN® MycoX Removal Mix to restore your cultures to their original, uncontaminated state, allowing you to move forward with your research without disruptions.